Paclitaxel 紫杉醇 天然抗肿瘤药

 Paclitaxel 紫杉醇 是一种天然抗肿瘤药，可稳定微管蛋白 (tubulin) 的聚合。Paclitaxel 可导致有丝分裂停滞和诱导细胞凋亡 (apoptosis)，最终导致细胞死亡。Paclitaxel 还可诱导细胞自噬 (autophagy)。

产品描述：
Paclitaxel (Taxol) is a naturally occurring antineoplastic agent and stabilizes tubulin polymerization. Paclitaxel can cause both mitotic arrest and apoptotic cell death. Paclitaxel also induces autophagy[1][2].

靶点：IC50 & Target     ：Traditional Cytotoxic Agents

体外实验:

Paclitaxel (20 nM; 48 hours) induces programmed cell death and exists a block at the G2/M phase of the cell cycle[1].
Paclitaxel (20 nM; 48 hours) induces a consistent increase in the level of p53[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]
Cell Line:     MCF-7, MDA-MB-231 cells
Concentration:     20 nM
Incubation Time:     48 hours
Result:     Induced programmed cell death.

Cell Cycle Analysis[1]
Cell Line:     MCF-7, MDA-MB-231 cells
Concentration:     20 nM
Incubation Time:     48 hours
Result:     >60% of MCF-7 cells and 50% of MDA-MB-231 cells were in the G2/M phase following 24 h treament.

Western Blot Analysis[1]
Cell Line:     MCF-7 cells (harboring wild-type p53)
Concentration:     20 nM
Incubation Time:     48 hours
Result:     Induced a consistent increase in the level of p53.

体内实验：
Paclitaxel (1-20 mg/kg; i.p.; 1 time/2 days for five cycles) obviously induces liver metastases at the low-Paclitaxel group with little influence on primary tumor growth[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:     MDA-231 xenograft-bearing mice[3]
Dosage:     1, 20 mg/kg
Administration:     Intraperitoneal injection; five cycles (1 time/2 days)
Result:     Liver metastases were obviously induced in the low-PTX (1 mg/kg) group with little influence on primary tumor growth compared with high-PTX group.